An instrument sterilization monitoring system is provided in the present application. The instrument sterilization monitoring system includes a server, a sterilization device, and a mobile device. The user uses the image capture module of the mobile device to capture a first indication device image information of a first indication device in the sterilization device, a first indication label image information of a first indication label, and a third indication device image information of the third indication label, a third indication label image information of a third indication device disposed outside the device packaging combination. The user uploads the image information to a server. The server determines whether the device packaging combination meets a sterilization standard based on the image information.本創作公開一種器械滅菌監測系統。器械滅菌監測系統包括伺服器、滅菌裝置以及移動裝置。使用者利用移動裝置的影像擷取模組擷取滅菌裝置中的第一滅菌裝置指示裝置的第一滅菌裝置指示裝置影像資訊以及第一滅菌裝置指示標籤的第一滅菌裝置指示標籤影像資訊、器械包裝組合外側的第二器械指示裝置、第二器械指示標籤的第二器械指示裝置影像資訊、第二器械指示標籤影像資訊,並將該些影像資訊上傳到伺服器。伺服器根據該些影像資訊判斷器械包裝組合是否符合一滅菌標準。