The Board of Trustees of the Leland Stanford Junior University
发明人:
Karl A. Deisseroth,Li Ye,Charu Ramakrishnan,Kimberly R. Thompson
申请号：
US16303903
公开号：
US20200318079A1
申请日:
2017.05.23
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The present disclosure provides nucleic acid activity-dependent expression vectors and activity-dependent expression cassettes for the activity-dependent expression of an encoded polypeptide. Also provided are recombinant adenoassociated viruses (AAV) containing an expression vector comprising an activity-dependent expression cassette for the activity-dependent expression of an encoded polypeptide by cells infected with the AAV vector. The present disclosure also provides methods for the activity-dependent labeling of cells in vitro or in vivo by introducing into the cells an expression vector containing an activity-dependent regulatory sequence driving expression of a labeling polypeptide. Also provided are methods for the activity-dependent control of cells in vitro or in vivo by introducing into the cells an expression vector containing an activity-dependent regulatory sequence driving expression of a light-responsive polypeptide.
