Disclosed herein are two-component enzymatic peracid generation systems and methods of using such systems wherein the first component comprises a formulation of at least one enzyme catalyst having perhydrolysis activity, a carboxylic acid ester substrate, and a cosolvent and wherein the second component comprises a source of peroxygen in water. The two components are combined to produce an aqueous peracid formulation useful as, e.g., a disinfecting or bleaching agent. Specifically, organic cosolvents are used to control the viscosity of a substrate-containing component and to enhance the solubility of the substrate in an aqueous reaction formulation without causing substantial loss of perhydrolytic activity of the enzyme catalyst.例えば、消毒剤および/または漂白用途での使用に好適なペルオキシカルボン酸の水溶液を酵素的に生成するための多成分調合物が本明細書に開示される。多成分ペルオキシカルボン酸調合物は、過加水分解活性を有する少なくとも1つの炭水化物エステラーゼ族7酵素を含む。