A tool for inserting a cylinder of a penile prosthesis into a patient includes a handle extending between first and second guides. The first guide is located at a first end portion of the tool and includes a first cylinder cradle on a first side of the tool and a first tubing cradle on a second side of the tool that is opposite of the first side of the tool. The second guide located at a second end portion of the tool and includes a second cylinder cradle on one of the first side or the second side of the tool and a second tubing cradle on the other side of the tool. The lateral width of the first tubing cradle and the second tubing cradle is the same and a lateral width of the first cylinder cradle is smaller than a lateral width of the second cylinder cradle.