MONSANTO TECHNOLOGY LLC;FORAGE GENETICS INTERNATIONAL LLC
发明人:
WHALEN DAVID,TEMPLE STEPHEN,MCCASLIN MARK,HIATT WILLIAM,REDDY MARRY S.,CERNY RICHARD ERIC
申请号：
NZ62919713
公开号：
NZ629197A
申请日:
2013.06.26
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is a method to select an alfalfa plant with enhanced feed value components from a population of alfalfa plants, wherein said population of alfalfa plants comprises a recombinant DNA molecule and wherein said recombinant DNA molecule has at least about 90% sequence identity with SEQ ID NO:1 or SEQ ID NO:2, comprising: (a) measuring the concentration of acid detergent lignin (ADL), guaiacyl lignin (G-lignin), and neutral detergent fibre digestibility (NDFD) in a lower stem tissue from each of said alfalfa plants in said population of alfalfa plants； (b) selecting an alfalfa plant that comprises the following enhanced feed value components: (i) a concentration of ADL reduced between 12% and about 31% compared to a lower stem tissue of a control alfalfa plant not comprising said DNA molecule； (ii) a concentration of G lignin reduced by about 25% or greater in said lower stem tissue compared to a lower stem tissue of a control alfalfa plant not comprising said DNA molecule； and (iii) a NDFD value increased by greater than 18% in said lower stem tissue compared to a lower stem tissue of a control alfalfa plant not comprising said DNA molecule； and (c) growing the alfalfa plant selected in step (b).
