A seedling transplanting mechanism for an automatic seedling supply apparatus is disclosed, which is basically arranged on a carrier that is capable of controlling travel speed and travel direction and comprises at least one plug-tray holding module, at least one seedling engagement module, at least one seedling move-out module, at least one seedling implanting module and a control module. In a travel process of the carrier, the control module is utilized to integrally coordinate movements of the plug-tray holding module, the seedling engagement module, the seedling move-out module and the seedling implanting module, thus to automatically implant a seedling of a plug tray to a farmland.本發明之一種自動供苗設備之種苗移植機構,基本上係於一可供控制行走速度及方向的載具上設有:至少一穴盤承接模組、至少一種苗銜接模組、至少一種苗移出模組、至少一種苗植入模組,以及一控制模組;使於載具行走的過程中,由控制模組整合穴盤承接模組、種苗銜接模組、種苗移出模組、種苗植入模組動作,達到自動將穴盤中之種苗植入田畦之目的。30‧‧‧載具31‧‧‧座椅32‧‧‧輔助輪33‧‧‧動力輪34‧‧‧方向機35‧‧‧動力系統36‧‧‧線性滑座37‧‧‧第六驅動組件38‧‧‧層架40‧‧‧穴盤承接模組41‧‧‧第一輸送帶411‧‧‧滾籠412‧‧‧履帶50‧‧‧苗銜接模組60‧‧‧種苗移出模組62‧‧‧第三驅動組件70‧‧‧種苗植入模組80‧‧‧種苗暫存模組90‧‧‧控制模組