JOSHI, Neel S.,NGUYEN, Peter Q.,DURAJ-THATTE, Anna,PRAVESCHOTINUNT, Pichet
申请号：
EP16777174
公开号：
EP3280397A4
申请日:
2016.04.06
申请国别(地区):
EP
年份:
2018
代理人:
摘要：
Embodiments of the present disclosure are directed to methods of genetically modifying bacteria to create amyloid-based materials, such as biofilms created by amyloid fibers, having nonnative functional polypeptides expressed thereon and connected thereto by a linker domain optimized for functioning of the non-native functional polypeptides. According to one aspect, the linker domain is optimized for functioning of a CsgA protein as is it assembled into an amyloid state and for functioning of the functional polypeptide. Exemplary biofilms may include living bacterial cells, non-living bacterial cells or combinations of living bacterial cells and non-living bacterial cells. Methods of making biofilms having non-native functional polypeptides attached thereto are provided.
