The invention provides a composite bacterial cellulose film, and method for manufacturing the same. A two-stage culture fermentation step is performed on a bacterial group. Purification and cleaning steps are performed to form a bacterial cellulose. The bacterial cellulose is then smashed. A glucose-amine and a polysaccharide are provided. The glucose-amine and the polysaccharide are uniformly mixed in a first predetermined ratio to form a mixed solution. The smashed bacterial cellulose is then mixed into the mixed solution in a second predetermined ratio. A frozen and dry step is performed to form a composite bacterial cellulose film. The above-mentioned two-stage culture fermentation step comprises a first-stage culture fermentation step and a second-stage culture fermentation step, wherein the temperature of the second-stage culture fermentation is higher than the temperature of the first-stage culture fermentation. The composite bacterial cellulose film of the present invention has good hemostatic and antibacterial characters.本發明提供一種複合生物纖維膜及其製造方法，包括：提供菌種；進行二階段醱酵培養；進行純化及清洗程序，形成生物纖維素，然後打碎生物纖維素；同時，提供葡萄糖胺與多醣體，將其以第一特定比例均勻混合，形成混合溶液；然後，將上述打碎之生物纖維素以第二特定比例均勻分散於混合溶液中；進行凍乾程序；最後形成複合生物纖維膜敷料。其中，上述二階段醱酵培養，包括：第一階段醱酵培養；以及第二階段醱酵培養，第二階段醱酵培養的溫度較第一階段醱酵培養的溫度為高。本發明之複合生物纖維膜具有極佳之止血性與抗菌性。
