A biological information acquisition device selects a light emitting unit located above a blood vessel as a measurement light emitting unit, and selects a light receiving unit spaced apart from the measurement light emitting unit by a predetermined distance and located above the blood vessel as a measurement light receiving unit and causes the measurement light receiving unit to acquire a first light receiving result. The device selects a light emitting unit which is not located above the blood vessel as a reference light emitting unit, and selects a light receiving unit spaced apart from the reference light emitting unit by the predetermined distance and not located above the blood vessel as a reference light receiving unit and causes the reference light receiving unit to acquire a second light receiving result.
