Tao ZHU,Haiyan CUI,Weiwei CHEN,Lei DUAN,Junqiang LI,Jin MA,Chunlin XIN,Zhongqi SHAO,Xuefeng YU,Huihua MAO
申请号：
US16806013
公开号：
US20200255862A1
申请日:
2020.03.02
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Provided are a cell strain HEK293.CS for reducing the production of a replication competent adenovirus, and a construction method and the use thereof. HEK293.CS is a safe adenovirus-producing cell line constructed by knocking out a gene fragment homologous to the Ad5 adenovirus E1 gene in HEK293 and providing a template plasmid to replace said gene fragment with a non-homologous sequence that stabilizes the expression of the E1 gene. Compared with the unmodified HEK293 cell strain, HEK293.CS shows no decrease in growth ability and virus production ability, but does not produce a detectable RCA. HEK293.CS can be used for the mass culture of a recombinant human type 5 adenovirus, and reducing the probability of RCA production in the manufacture process of drugs such as vaccines and antibodies.
