FIEBIG, HELMUT, PROF.,NANDY, ANDREAS, DR.,CROMWELL, OLIVER, DR.
申请号：
EP10009677.5
公开号：
EP2261336B1
申请日:
2004.12.01
申请国别(地区):
EP
年份:
2013
代理人:
摘要：
Die vorliegende Erfindung betrifft die Bereitstellung von DNA-Sequenzen von Gruppe-4 Majorallergenen aus Getreiden. Die Erfindung schließt auch Fragmente, Neukombinationen von Teilsequenzen und Punktmutanten mit hypoallergener Wirkung ein. Die rekombinanten DNA-Moleküle und die abgeleiteten Polypeptide, Fragmente, Neukombinationen von Teilsequenzen und Varianten können zur Therapie von pollenallergischen Krankheiten genutzt werden. Die rekombinant hergestellten Proteine können zur In-vitro-und In-vivo-Diagnostik von Pollenallergien eingesetzt werden.DNA molecules (I), corresponding to the sequence of the major allergens of cereal pollen are new. (I) have sequences (1 1603 nucleotides (nt)) (3 1644 nt) (5 1608 nt) (7 1603 nt) or (9 1603 nt), all reproduced. DNA molecules (I), corresponding to the sequence of the major allergens of cereal pollen comprise sequences comprising 1603 nucleotides (SEQ ID No.:1) (nt)) 1644 nucleotides (SEQ ID No.:3 ) 1608 nucleotides (SEQ ID No.:5 ) 1603 nucleotides (SEQ ID No.:7 ) or 1603 nucleotides (SEQ ID No.:9 ). Independent claims are also included for: (1) DNA (Ia) that hybridizes to (I) under stringent conditions and is derived from DNA of Poaceae (2) DNA (Ib) that encodes a polypeptide that is immunologically cross-reactive with the major allergens Sec c 4 ( Secale cereale) Hor v 4 ( Hordeum vulgare) or Tri a 4 ( Triticum aestivum) and derived from Poaceae (3) DNA fragment, or combination of fragments, of (I), (Ia) or (Ib) that encodes an immunomodulatory T-cell reactive fragment (X) of Group 4 Poaceae allergens (4) a DNA corresponding to any of the new DNAs or fragments that encodes an (X) but is modified by targeted mutation of individual codons, deletion or addition (5) recombinant DNA expression vector or cloning system containing any of the new DNAs linked to expression control sequences (6) host organism transformed with any of the new DNAs or the vector of (5) (7) preparing a polypeptide (II) encoded by the new DNAs by cultu
