An instrument access device comprises a retractor assembly including a distal anchoring member (11) for insertion into a wound interior and a proximal member (23, 24) for location externally of a wound opening. An elongate member (12) extends between the distal member (11) and the proximal member (23, 24) to retract laterally the sides of a wound opening, The device comprises a seal or valve (70) and a flexible connector (75, 80) extending between the proximal member and the instrument seal or valve (70).