The present disclosure relates to a bioprinter spray head assembly and a bioprinter, wherein the bioprinter spray head assembly comprises an outer nozzle having a second channel and an inner nozzle having a first channel, the inner nozzle is coaxially provided within the second channel, the first channel forms a first material channel, and an annular space between the outer nozzle and the inner nozzle forms a second material channel, which surrounds the first material channel at an outlet of the first material channel, for converging a second material sprayed from an outlet of the second material channel toward a first material sprayed from the outlet of the first material channel, so as to form a fluid printing unit. Such spray head assembly can allow that the second material uniformly wraps the first material at the outlet of the spray head assembly, so as to form a fluid printing unit having a high quality to protect the cells, thereby reducing the cell damage caused by the extrusion pressure and the frictional force subjected in the printing process, so as to improve the survival rate of the cells.