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COMPOSITIONS ET PROCÉDÉS COMPRENANT DES AMÉLIORATIONS D'ARN GUIDES DE CRISPR À L'AIDE DU PROMOTEUR H1
专利权人:
The Johns Hopkins University
发明人:
JASKULA-RANGA, Vinod,ZACK, Donald,WELSBIE, Derek, S.
申请号:
EP17824801
公开号:
EP3481956A4
申请日:
2017.07.05
申请国别(地区):
EP
年份:
2020
代理人:
摘要:
The presently disclosed subject matter provides compositions and methods comprising improvements of a CRISPR system (e.g. CRISPR associated (Cas) 9 (CRISPR-Cas9, non-Cas9 CRISPR systems). Such compositions may comprise modifications to the H1 promoter region, addition of 5′UTR modifications, different orthologous sequences of the H1 promoter, novel compact bidirectional promoter sequences with both pol II and pol III activity, addition of Kozak consensus sequences, termination sequences, addition of conditional pol II/pol III bidirectional promoter expression, addition of a donor template sequence for correcting mutations, or combinations thereof. Other aspects of the invention relate to modifications to Cas9 through post-transcriptional cell-cycle regulation fusions, engineered partial target sites such that the nuclease can bind without DNA cleavage, auto-regulation sites, and N-terminal modifications to modulate half-life.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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