#$%^&*AU2015101218A420151008.pdf#####Abstract A cell-scaffold complex and 3D printing fabrication method comprises a biological scaffold resemble with actual defects of autologous organs or tissues, nanofibrous layers printed on the biological scaffold capable of mimic natural extracellular matrix, which are beneficial to cells for adhering to, growing and proliferating and cell suspension printed on the nanofibrous layers. The required cell-scaffold complex can be accurately printed in accordance to the actual defects of patients organs or tissues through the 3D printing fabrication method.