LOREY, SUSAN,JANOWSKI, BERNHARD,PULTKE, HEIKO,KATHMANN, DANIELA,PARTHIER, ANTJE,ANTON, ANDREAS
申请号：
RSP20180159
公开号：
RS56893B1
申请日:
2012.12.19
申请国别(地区):
RS
年份:
2018
代理人:
摘要：
The present invention relates to a proNGF mutant and to uses thereof, in particular the use of a proNGF mutant for producing human beta-NGF. The present invention discloses a method of preparing a biologically active human beta-NGF from an inactive insoluble proNGF mutant. A proNGF mutant of the invention is substituted by any amino acid but not Arg or Lys at the native protease cleavage site R 1 SK 3 R 4 at least at positions R 1 and K 3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence.
