A use of an orchid callus extract in the manufacture of a composition is provided, wherein the extract is a polar solvent extract of a callus of orchid leaves, and the composition is used for protecting skin, especially for moisturizing skin, increasing skin tenderness, increasing skin abundance, increasing skin elasticity, tightening skin, reducing skin textures, delaying skin aging, assisting in increasing content of collagen, repairing skin tissues, preventing skin lesions, and/or promoting wound healing.一種使用蘭花癒傷組織萃取物於製備一組合物之用途，其中該萃取物係一蘭花之葉片癒傷組織的極性溶劑萃取物，且該組合物係用於護膚，尤其是用於保濕、提升皮膚柔潤度、提升皮膚飽滿度、提升皮膚彈性、緊緻皮膚、減少皮膚紋理、延緩皮膚老化、幫助提升皮膚膠原蛋白含量、修復皮膚組織、預防皮膚病變、及／或促進傷口癒合。
