A flexible port seal is provided for use in single incision surgery and includes an outer seal defining a bore extending from a proximal end of the outer seal to a distal end of the outer seal. A support plate is located within the bore of the outer seal and one or more throughports are attached to the support plate for receipt of a surgical instrument. The throughports form a fluid tight seal with cannulas and/or surgical instruments inserted through the throughports. The flexible port seal is formed of a compressible or flexible material to facilitate insertion through the single incision in the patient and allow for movement of the surgical instruments relative to the seal to independently orient each of the surgical instruments.
