A system 200 for aligning a tool in relation to a trajectory in real-time which comprises a processor capable of receiving input data from a pre-operative plan image, at least one multi-modal image, and at least one real-time multi-modal image. The system interactively tracks at least one neural fiber and generates output data 205a by way of data transformation using the input data and the interactively tracked neural fiber data. The output data 205a is transmitted to at least one of at least one display device 211 for rendering at least one real-time interactive navigation display for facilitating neural navigation, and at least one drive device for positioning at least one tracking device in relation to the tool in real-time, whereby real-time alignment data is achievable, and whereby at least one neurological structure is preservable. The drive device may comprise an arm 202, the arm may be robotic. The display device may comprise an interactive window. The system may comprise a tracking device which may be formed from an optical camera, radio frequency tracking device or an electromagnetic tracking device. The real-time interactive navigation display may display an indicia such as textual information, navigation symbol or an alignment symbol.
