The present invention provide cheap and liquid strain bacterium efficiently manufacturing method in a kind of extensive business manufacture of mushroom class fructification, using the liquid strain bacterium mushroom class fructification manufacturing method. A kind of manufacturing method of the liquid strain bacterium of mushroom class of what is closed in system of the present invention, and characteristic is in what: it is to manufacture mushroom class fructification person, and what contains in the fluid nutrient medium of vegetable oil, carries out the culture of liquid deep by using bubble agitation culture medium. Also, the present invention also closes a kind of manufacturing method of mushroom class fructification of what, characteristic is in what: in the cultivation of what bottle, using the further bacterium bed cultivation culture medium for increasing bacterium seat. By the present invention, kind of a bacterium manufacturing time can be greatly shortened, in addition, the risk of manufacture unevenness when can avoid using solid kind bacterium manufacture mushroom class fructification, and can steadily manufacture excellent mushroom class fructification.本發明提供一種菇類子實體之大規模之商業製造中之廉價且高效之液體種菌之製造方法、使用該液體種菌之菇類子實體之製造方法。本發明係關於一種菇類之液體種菌之製造方法，其特徵在於：其係用以製造菇類子實體者，並且於含有植物油之液體培養基中，藉由使用氣泡攪拌培養基而進行液體深部培養。又，本發明亦關於一種菇類子實體之製造方法，其特徵在於：於瓶栽培中，使用進一步升高菌座之菌床栽培用培養基。藉由本發明，可大幅度縮短種菌製造時間，又，可避免使用固體種菌製造菇類子實體時之製造不均之風險，並且可穩定地製造優良之菇類子實體。
