A method of manufacturing a tissue fastening tool for fastening in close contact with the living tissue of two, includes the following steps. Step of securing the shaft member one end of the wire made of metal, while twisting the wire around the axis of the wire, and forming the coil by winding the wire on the outer circumference of the shaft body. Performing a heat treatment for imparting superelasticity to the wire. step of the opposite winding direction of the coil wound in the process the wire rod is deformed in an elastically deformable range, the wire superelastic has been applied, forming the coil winding direction of the coil. In this manufacturing method, after the step of reversing the winding direction of the coil, the initial tension to the compression direction of the coil is applied to the coil.2つの生体組織を密着させて締め付ける組織締付具の製造方法であって、以下の工程を含む。金属からなる線材の一端を軸体に固定し、前記線材の軸回りに前記線材をねじりつつ、前記軸体の外周に前記線材を巻いてコイルを成形する工程。前記線材に超弾性を付与する熱処理を行う工程。超弾性が付与された前記線材を、前記線材が弾性変形可能な範囲で変形させて、前記コイルの巻き方向を前記コイルを成形する工程で巻いた前記コイルの巻き方向と逆にする工程。この製造方法においては、前記コイルの巻き方向を逆にする工程の後に、前記コイルの圧縮方向への初張力が前記コイルに作用している。
