An ultrasound diagnostic apparatus which detects a target blood vessel of a subject based on reflected ultrasound waves obtained, using an ultrasound probe, from the subject, the ultrasound diagnostic apparatus including: a B-mode image generation unit which generates a tomographic image of the subject, based on the reflected ultrasound waves a blood flow image generation unit which generates blood flow information indicating a blood flow region of the subject in the tomographic image, based on the reflected ultrasound waves and a blood flow region determination unit which determines whether or not the blood flow region corresponds to the target blood vessel, by analyzing the blood flow information generated by the blood flow image generation unit.