A test cup (1) for analysing a fluid specimen includes a bottom (2), a wall (3) extending from the bottom (2) and an opening (5) opposite the bottom (2), the bottom (2) and the wall (3) defining a cavity with an inner side (6) for accommodating a fluid specimen containing an analyte. A test region (10) is arranged on or at the bottom (2) and contains a binding partner for the analyte, wherein a reaction of the binding partner with the analyte causes a change of a colour intensity of at least a portion of the test region (10) when the test region (10) comes in contact with the analyte of the fluid specimen. At least one of a colour calibration reference element (12) for a colour based calibration and a location reference element (11) for a location calibration, is arranged on or at the bottom (2). The test region (10) and at least one of the location reference element (11) and the colour calibration reference element (12) are arranged on or at the bottom (2) such that they are in the same field of view (102) of a camera (101), when the camera (101) is placed in a distance to the bottom (2) and is facing the bottom (2).