To provide a method in which mononuclear cells are differentiated into a good balance of NK cells, NKT cells, and T cells, and said cells are made to proliferate. The present invention provides a method for manufacturing an immune cell-containing composition, said method including the following steps: a first step in which mononuclear cells are cultured in a medium containing anti-CD16 monoclonal antibodies and either IL-2 and/or IL-15 and a second step, after the first step, in which culturing is performed under conditions that make the mononuclear cells preferentially differentiate into cytotoxic T cells. This method may also include a third step, after the second step, in which culturing is performed in a medium containing anti-CD16 monoclonal antibodies and either IL-2 and/or IL-15.本發明提供一種使單核細胞非常均衡地分化成NK細胞、NKT細胞和T細胞,並使該等細胞增殖之方法。本發明提供一種含免疫細胞之組成物的製造方法,包含以下步驟:第1步驟,於含有IL-2及/或IL-15、和抗CD16單株抗體之培養基中培養單核細胞;及,第2步驟,於上述第1步驟後,在使單核細胞優先分化成細胞毒性T細胞之條件下進行培養。於上述第2步驟後亦可包含第3步驟,該第3步驟是於含有IL-2及/或IL-15、和抗CD16單株抗體之培養基中進行培養。
