A target path determining method, applied to a determining system. The determining system comprises a photography apparatus (1) and a positioning apparatus (3) separated from each other, and a calibration apparatus (2) connected to the photography apparatus (1). The method comprises: S1. obtaining a three-dimensional local image for an affected part and a virtual path in the three-dimensional local image； S2. matching a simulated two-dimensional image obtained by projection on the basis of the three-dimensional local image and a two-dimensional projection image obtained on the basis of the affected part； S3. if the simulated two-dimensional image is matched with the two-dimensional projection image, determining, according to the position information of the virtual path on the simulated two-dimensional image, a surgery guiding path corresponding to the virtual path on the two-dimensional projection image； and/or S4. if the simulated two-dimensional image is matched with the two-dimensional projection image, obtaining three-dimensional pose information of the virtual path on the three-dimensional local image； and matching the direction of a guidance structure (32) with the surgery guiding path according to a second conversion relationship among perspective coordinate information, a perspective coordinate system, and a positioning coordinate system based on the positioning apparatus (3).La présente invention concerne un procédé de détermination d'un trajet cible, appliqué à un système de détermination. Le système de détermination comprend un appareil photographique (1) et un appareil de positionnement (3) séparés l'un de l'autre, et un appareil de calibrage (2) relié à l'appareil photographique (1). Le procédé comprend : S1. l'obtention d'une image locale tridimensionnelle pour une partie affectée et d'un trajet virtuel dans l'image locale tridimensionnelle ； S2. la correspondance d'une image bidimensionnelle simulée obtenue par la projection sur la base de l'image loc
