JOSEPH GERARD RAKESH RAJ JOSEPH SURESH,JOSEPH GERARD RAKESH RAJ JOSEPH SURESH
申请号：
IN684/CHE/2014
公开号：
IN2014CH00684A
申请日:
2014.02.13
申请国别(地区):
IN
年份:
2015
代理人:
摘要：
This invention relates to identification of bio-active phyto constituents from medicinal plants which have activity on macrofilaricidal. Methanolic plant extracts of medicinal plants were screened at Northwick Park Institute of Medical Research,(NPIMR), London, U.K., on human filarial adult male worm Onchocerca gutturosa. Four plant extracts were found to be effective in the in vitro screening based on mean motility, and the viability of adult using 3-[4,5-dimethylthiazol-2yl}-2,5-diphenyl tetrazolium bromide (MTT). The mean motility was 0 and the motility reduction was 100%. In the in vivo test on O.lienalis (skin mf) in CBA/CA mice at the dose of 100mg/Kg (s.c) for five consecutive days, it was observed that the mf worm reduction was <； 46.77. These extracts were sent to Central Drug Research Institute, Lucknow, India for testing in vitro and in vivo on B. malayi animal model for adulticidal activity. One extract was found to be active against adult B. malayi exhibiting MIC (Minimum Inhibitory Concentration) of 31.25 µg/ml and IC50 (50% inhibitory concentration) 9.4 µg/ml. SI (Selectivity index) value was 10.6. As the SI was >； 10, it was considered safe for further in vivo evaluation. The in vivo test carried on Jirds model the adulticidal activity over control was 54.5±5.2. The predominant bioactive phyto chemicals of Datura metel were identified as (1) Atropine and (2) Scopolamine. The Central Drug Research Institute, Lucknow confirmed that the Atropine has shown 23.5% adulticidal effect and we expect 60% adulticidal effect in scopolamine. The chemical structure of the Atropine and Scopolamine are as under:
