An in-vitro biological valve calcification evaluation method, comprising the following steps: preparing calcification evaluation solution comprising glutaraldehyde solution, saline solution, borate buffer solution, reducing calcium ingredient solution and calcium-containing plasma solution, the reducing calcium ingredient solution comprising alcohol, alkaline solution, oil and its derivatives, and buffer solution； preheating the reducing calcium ingredient solution； conducting treatment with the glutaraldehyde solution prior to reducing calcium ingredient solution； conducting treatment with the reducing calcium ingredient solution； preserving the valve samples； conducting in-vitro calcification reaction with the calcium-containing plasma solution； and detecting calcium content by atomic absorption spectrum.
