LOURDES GEORGINA IGLESIAS ANDREU,JERICO JABIN BELLO BELLO,ALMA LAURA RAMOS CASTELLA
申请号：
MX2013008201
公开号：
MX362298B
申请日:
2013.07.15
申请国别(地区):
MX
年份:
2018
代理人:
摘要：
The present invention refers to the development of an efficient process for the micropropagation of vanilla. Vanilla (Vanilla spp.) is an orchid commercially cultivated since this produces vanilla, a flavoring substance widely demanded in the food and cosmetic industry. Nowadays, this specie is commercially spread by the asexual via by means of setts, which are frequently obtained from the same commercial plantations. This propagation method has contributed to exhibit serious problems of endogamy, which are negatively affecting the proper commercial exploitation thereof. Considering the aforementioned problem as well as the important lack of vegetative propagules that is being produced in this cultivation, it is important to have an efficient process for the commercial propagation of this species. The present invention therefore proposes an efficient process for the micropropagation of vanilla at a commercial scale using a system of Containers of Automated Immersion. The process compr ises the following stages: Stage 0. Selection and pretreatment of mother plants. Stage 1. Establishment of aseptic crops, Stage 2. Multiplication in vitro, Stage 3. Elongation and rooting and Stage 4. Acclimatization.La presente invención se refiere a un proceso para aumentar la tasa de multiplicación de brotes por explante de Vanilla spp mediante micropropagación in vitro, que comprende las siguientes etapas: Etapa 0. Selección y pre-tratamiento de plantas madre Etapa 1. Establecimiento de los cultivos asépticos Etapa 2. Multiplicación in vitro Etapa 3. Elongación y enraizamiento Etapa 4. Aclimatación caracterizado porque la etapa de multiplicación in vitro, se lleva a cabo en un biorreactor de inmersión temporal a una frecuencia seleccionada entre 4, 8, y 12 hrs por un tiempo de inmersión de al menos 2 min, con un volumen del medio de cultivo de 25-50 ml por explante, durante al menos 30 día y en donde la etapa de elongación y enraizamiento se realiza cuando los brotes presentan una
