A suturing method includes inserting an endoscope having a pressing part attached to a distal end thereof into a body cavity, and moving the endoscope to a near side of tissue inserting a suturing instrument having a suturing unit mounted thereon into a channel of the endoscope, and projecting a distal part from the distal end of the endoscope moving the anchor holder having the plurality of anchors accommodated therein to a back side opposite to the near side of the tissue and inserting the distal end of the anchor holder into the tissue from the back side opposite to the near side while the tissue is pressed by the pressing part from the near side, and ejecting at least one of the plurality of anchors from the anchor holder which is attached to ends of a suture thread in the suturing unit.
