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Creation of antibodies that function in cells and identification of proteins that control cell phenotypes
专利权人:
独立行政法人産業技術総合研究所
发明人:
井上 敦,ワダワ レヌー,吉崎 慎矢
申请号:
JP2006328529
公开号:
JP5105468B2
申请日:
2006.12.05
申请国别(地区):
JP
年份:
2012
代理人:
摘要:
<;P>;PROBLEM TO BE SOLVED: To provide a gene therapeutic composition against malignant cancer cells by identifying a factor deciding the phenotype of cells such as the cancer cells and obtaining an intracellular antibody specific to the factor, and also an expression library and a method for screening the same. <;P>;SOLUTION: This intracellular antibody inhibiting (activating) the change of phenotype of the cells is obtained by a screening method comprising a process of constructing a heavy chain variable region (VHH) expression library by randomizing the part of sequence of the VHH of a camel heavy chain antibody, and determining the sequence of the randomized part by isolating the VHH in the cells of expressing the change of the phenotype in cultured cells transformed by using the expression library. By using the intracellular antibody, it is possible to identify an intracellular physiologically active substance controlling the phenotype of the cells, and provide a gene therapeutic composition of the cancer by using a vector expressing the intracellular antibody. <;P>;COPYRIGHT: (C)2008,JPO&INPIT
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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