A detection device for an acupuncture point and a detection method using the same are provided. The detection method includes the following steps. Firstly, an ultrasonic probe slightly touches a human body and sends a detection signal to the human body, wherein the detection signal is reflected to become a first reflection signal. Then, the ultrasonic probe applies a pressure to the human body and sends the detection signal to the human body, and the second pressure is larger than the first pressure. Then, a processor determines information of the acupuncture point according to the first reflection signal and the second reflection signal.一種穴點偵測裝置及應用其之穴點偵測方法。穴點偵測方法包括以下步驟。首先,超音波探頭輕觸在人體上並對人體發出偵測訊號,其中偵測訊號自人體反射後成為第一反射訊號。然後,超音波探頭對施壓在人體上並對人體發出偵測訊號,其中偵測訊號自人體反射後成為第二反射訊號。然後,處理單元比較第一反射訊號與第二反射訊號,據以判斷人體之一穴點的資訊。S110、S120、S130、S140‧‧‧步驟
