Jean-Pascal Pierre Bilgischer;Mark Robert Pearce-Higgins;Andrew John Kenny;Philip Jonathan Bassett
发明人:
Jean-Pascal Pierre Bilgischer,Philip Jonathan Bassett,Mark Robert Pearce-Higgins,Andrew John Kenny
申请号：
US13576980
公开号：
US10487136B2
申请日:
2011.02.02
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present disclosure relates to a method for the manufacture of recombinant antibody molecules comprising culturing a host cell sample transformed with an expression vector encoding a recombinant antibody molecule； adding an extraction buffer to the sample； and subjecting the sample to a heat treatment step； wherein the pH of the sample is detected after addition of the extraction buffer, and optionally adjusted, to ensure that the pH of the sample is 6 to 9 prior to the heat treatment step.
