Microdialysis method and apparatus of the present invention can be used to examine the metabolism surface of organs of the human and animals. Microdialysis catheter, for example, when placed in the heart beating beating is concerned the problem of the position of the catheter. Further, there is a risk of damaging cardiac tissue and catheter. The present invention provides such it is designed to avoid damage, data obtained from epicardial probe of the present invention it was confirmed that reflects the metabolic state of the myocardium. For anesthetized of normal ventilation state pigs (n = 20), around the diagonal branch of the left anterior descending (LAD) artery performs a sternotomy, arranged the snare of the Gore-Tex sutures, one of the microdialysis probe, snare is inserted into the myocardial tissue supplied by the LAD artery was applied. Another microdialysis probe was placed above the epicardial surface of the myocardium probe. Protocol includes four 10-minute duration of ischemia (intervention group), were those subsequent 40 minutes of ischemia period followed. The control group, were exposed only to the 40-minute duration of ischemia. The microdialysis probes of the present invention arranged on epicardial surface, it was possible to recover the sample to reflect the myocardial metabolic state of the heart. It is also possible to measure fast changes in concentration, especially lactate, are eventually detectable increase in short ischemia-life of 10 minutes was observed. When analyzing the concentration of glucose and glycerol in the sample pairs, the same tendency was seen. Data acquired by the apparatus and method of the present invention disposed on the epicardial surface, when compared with the acquired data by placed normal probe into the myocardium showed the same tendency. Application of myocardial membrane according to the present invention is effective for intraoperative monitoring of cardiac surgery. .FIELD 9本発明の微小透析方法及び装置は、人間及び動物の臓器の代謝面について調
