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PRODUCTION OF POLIO VIRUS AT HIGH TITERS FOR VACCINE PRODUCTION
专利权人:
CRUCELL HOLLAND B.V.
发明人:
LEWIS JOHN ALFRED
申请号:
ZA201108271
公开号:
ZA201108271B
申请日:
2011.11.10
申请国别(地区):
ZA
年份:
2012
代理人:
摘要:
Provided is a process for the production of poliovirus, comprising the steps of: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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