PROBLEM TO BE SOLVED: To provide a method of manufacturing a single-wall cell layer such as a single-wall epithelial/endothelial cell layer from a stem cell, and a medical drug using the single-wall cell layer obtained by the method of the present invention.SOLUTION: A method of manufacturing a single-wall cell layer of differentiated cells by inducing a stem cell into differentiation includes: a process (first process) of culturing a stem cell on a culture medium containing a BMP/activin inhibitor and a process (second process) of subsequently culturing the stem cell on a culture medium containing the BMP/activin inhibitor and an Abl/PDGFR/c-kit inhibitor.COPYRIGHT: (C)2015,JPO&INPIT【課題】幹細胞から単層上皮・内皮細胞層等の単層細胞層を製造する方法及び本発明の方法により得られた単層細胞層を用いた医薬の提供。【解決手段】幹細胞を、BMP/アクチビン阻害剤を含む培地で培養する工程(第1工程)、続いてBMP/アクチビン阻害剤及びAbl/PDGFR/c-kit阻害剤を含む培地で培養する工程(第2工程)を含む、幹細胞を分化誘導して分化細胞の単層細胞層を製造する方法。【選択図】なし
