A single-use, extrusion apparatus and method of using the apparatus. The apparatus includes a first housing member having a first port dimensioned to receive a first needle-tipped syringe, and a first needle path dimensioned to receive a first needle, wherein the first needle path extends from the first port through the first housing member； a second housing member having a second port dimensioned to receive a second needle-tipped syringe, and a second needle path, dimensioned to receive a second needle, wherein the second needle path extends from the second port through the second housing member and is collinear with the first needle path extending through the first housing member； and a membrane filter disposed between the first and second housing members, wherein a total dead-volume of the apparatus, not including the first and second needle paths is less than or equal to about 50 microliters.
