A system and method for determining and managing the volume and concentration of sanitiser optimised for use in a method for disinfecting an intra-ovum substance application module, with information on the average size of the vaccinated and/or fed fertile eggs being used as the paradigm, represent a solution in the field of poultry farming, in particular in the poultry breeding sector, particularly useful to aid the disinfection of the components of operational modules of the breeding system, in particular the substance application module [E12], adding value in that it ensures that a volume of prepared concentrated sanitiser (Sa31) with assured quality is obtained, especially in that it exhibits titration with a concentration suitable for the batch of vaccinated/fed fertile eggs, ensuring the suitability and effectiveness in respect to the true mean age of the matrix batch, determined as a function of average egg size (Tm1), wherein for that purpose the system of the invention was designed with an architecture comprising the following modules: fertile egg batch data module (M1), action management module (M2), concentrated sanitiser module (M3), water purification module (M4) and module (M5) for preparing the prepared concentrated sanitiser.