Disclosed is a vascular image positioning system, including an image enhancing device (1) and an image projection device (2) used cooperatively with the vascular image enhancing device (1). The transmission light emitted by the enhancing device (1) passes through the sites of the human body surface to be tested, is received by the image projection device (2), and after image processing, forms in situ images at sites on the surface of the skin of the human body to be tested. The enhancing device (1) comprises a light emitting unit (101) and a drive unit (102) driving the light emitting unit (101) to emit light. The vascular image positioning system provides the function of using a transmission light source to achieve in situ imaging of the blood vessels at the sites to be tested; secondly, it also provides a mode for combining a transmission light source with a reflection light source, thereby strengthening the function of in situ imaging and widening the application range.