Рекомбинантный белок DMA-CBD, способ его получения, рекомбинантная плазмида pDMA-CBD и штамм E. coli M15 для его получения, инъекционный препарат для повышения мышечной массы сельскохозяйственных животных, птицы и животных семейства псовых, а также метод использования препарата
Юдин Сергей Михайлович (RU),Сергиенко Ольга Васильевна (RU),Лунин Владимир Глебович (RU)
申请号：
RU2014135790
公开号：
RU2014135790A
申请日:
2014.09.03
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. Recombinant protein DMA-CBD with a molecular weight (25,73 ± 1000) Yes pI 5,05, DMA protein comprising a fragment of sequence SEQ ID NO 1, Gly-Ser spacer to SEQ ID NO 2 sequence tsellyulozosvyazuyuschy domain endoglucanase gene CelD from A. thermophilum sequence with SEQ ID NO 4.2. The process for producing DMA-CBD on the cellulose recombinant protein comprising: growing cells of the strain E. coli M15 [pREP4, pDMA-CBD], DMA-CBD binding protein in cell extracts of E. coli M15 [pREP4, pDMA-CBD] Cellulosic sorbent due affinity interactions by incubation procedure, subsequent washing of unbound bacterial proteins ivydelenie produkta.3 target. The recombinant plasmid pDMA-CBD with the nucleotide sequence SEQ ID NO 4 provides DMA-CBD expression of a recombinant protein consisting of A determinants myostatin, Gly-Ser spacer domain endoglucanase gene tsellyulozosvyazuyuschego CelD from Anaerocellum thermophilum (A. thermophilum), size of 4091 n. n, consisting of the following structural elements: a. an artificial bacterial operon DMA-CBD recombinant protein comprising the promoter region of an early promoter of bacteriophage T5 7-87 bp DMA-CBD gene recombinant protein 115-813 bp nontranslated termination region trance RTOC 1693-1791 bp bacterial operon beta-lactamase, providing resistance to ampicillin 3886-3029 bp complementary strand, a bacterial origin of replication site type ColE1 2263-2273 bp provides plasmid replication in strains of E. coli.4. The strain of E. coli M15 [pREP4, pDMA-CBD], obtained by transformation with the plasmid of claim 3, deposited in the Russian Collection of Microorganisms under number VKM B-2880D.5. The injection for increasing muscle mass of farm animals, poultry and animals canine containing the recombinant protein DMA-CBD of claim 1, suspended in a medium of amorphous cellulose to 90% by weight, acceptably1. Рекомбинантный белок DMA-CBD с молекулярной массой (25,73±1000) Да pI 5,05, включающий фрагмент белка DMA с последовательностью S
