A manufacturing and separating device and a method for oily radioactive substances provide an automatic synthesis box to minimize the man-made errors during the manufacturing, such that mass production for the oily radioactive substance can be possible, the radiation exposure time of the manufacturing staff can be reduced, and the radioactivity of the final products can be detected. The manufacturing and separating device includes a first reaction material bottle, a first heating device, a gas pump, a first flushing material bottle, a second flushing material bottle, a purifying device, a first transmission unit, a temperature-controlled temporary storage tank, a filter, a first collection bottle, a second collection bottle, a plurality of valves and a control unit.
