Lance P. Encell;Keith V. Wood;Monika G. Wood;Michael R. Slater;Brock Binkowski;Mary Hall;Matthew B. Robers
发明人:
Brock Binkowski,Lance P. Encell,Mary Hall,Matthew B. Robers,Michael R. Slater,Keith V. Wood,Monika G. Wood
申请号：
US13287986
公开号：
US20120174242A1
申请日:
2011.11.02
申请国别(地区):
US
年份:
2012
代理人:
摘要：
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
