PROBLEM TO BE SOLVED: To provide a method for detecting peroxisomal fatty acid &beta-oxidation-based activated substances capable of enhancing peroxisomal fatty acid &beta-oxidation activity.SOLUTION: The method for detecting the peroxisomal fatty acid &beta-oxidation-based activated substances cincludes the steps of adding an agent to be detected and a labelled super long chain fatty acid to human skin fibroblasts reduced in the &beta-oxidation activity of super long chain fatty acids, culturing the same and measuring the labelled amount in the aqueous metabolized product after culturing.COPYRIGHT: (C)2010,JPO&INPIT【課題】 ペルオキシソーム脂肪酸β酸化系活性を向上することができるペルオキシソーム脂肪酸β酸化系活性化物質の検出方法の提供。【解決手段】 極長鎖脂肪酸のβ酸化活性が低下したヒト由来の皮膚繊維芽細胞に、被検試薬と末端が標識された極長鎖脂肪酸を加えて培養し、培養後の水性代謝物中の標識量を測定するペルオキシソーム脂肪酸β酸化系活性化物質の検出方法。【選択図】 図1
