To obtain a large amount of human fat tissue-derived somatic stem cells holding high differentiation potency to an extent where the cells can be practically used for regenerative medicine.SOLUTION: There is provided a manufacturing method of human fat tissue-derived somatic stem cells in which human fat tissue-derived cells attached to a solid phase are separated into cells which are easily detached from the solid phase and cells which are difficult to be detached from the solid phase, the separated easily detachable cells are cultured in low oxygen under 7% of oxygen concentration, by which a large amount of human fat tissue-derived somatic stem cells holding high differentiation potency can be obtained to an extent where the cells can be practically used for regenerative medicine.【課題】本発明は、高い分化能を保持したヒト脂肪組織由来体性幹細胞を、再生医療に実用できる程度に多くの量を得ることを目的とする。【解決手段】ヒト脂肪組織由来体性幹細胞の製造方法であって、固体相に付着したヒト脂肪組織由来の細胞を、固体相から剥離しやすい細胞と固体相から剥離しにくい細胞とに分離し、分離した剥離しやすい細胞を７％の酸素濃度下で低酸素培養することで、高い分化能を保持したヒト脂肪組織由来体性幹細胞を、再生医療に実用できる程度に多くの量を得ることができる。
