A method of micropatterning a substrate is provided. The method comprises providing a template substrate having a patterned surface inked with a composition of interest contacting the patterned surface with an intermediate substrate, thereby transferring the composition to the surface of the intermediate substrate contacting the surface of the intermediate substrate comprising the composition with the substrate and removing the intermediate substrate by dissolution using a solvent. A patterned substrate formed using the method, as well as a biosensor comprising the patterned substrate is also provided.