Usage: the photodynamic inactivation, on medical instruments, of viruses containing RNA and DNA. Aim: to develop an effective method, involving minimal outlay of effort and energy, for inactivating, on medical instruments, viruses containing RNA and DNA, and also an apparatus for implementing same by achieving maximal activation of a photochemical agent during interaction with monochromatic light. Essence of the invention: a method for inactivating, on medical instruments, viruses containing RNA and DNA, including the preliminary mechanical cleaning and washing of the instruments, then keeping the instruments in an aqueous solution of methylene blue with maximum activation taking place during the interaction of the solution with monochromatic light. In the method according to the invention, maximum activation is achieved in a solution of methylene blue having a concentration of 0.01-0.02% by means of interaction with light in the emission spectrum of monochromatic emitters having wavelengths ranging between 582 and 592 nm or between 658 and 662 nm and an overall light output of at least 280 lm. The instruments are kept in the solution for 90 minutes. An apparatus for inactivating, on medical instruments, viruses containing RNA and DNA comprises a hermetically-sealing housing with a chamber, an instrument container which is located in the chamber and which is filled with a solution of methylene blue, and, installed above the container, a source of monochromatic light. The apparatus according to the invention contains a source of monochromatic light comprising monochromatic emitters in an emission spectrum having wavelengths ranging between 582 and 592 nm or between 658 and 662 nm and an overall light output of at least 280 Im. LEDs are selected as the monochromatic emitters.