PROBLEM TO BE SOLVED: To provide a useful observation method of cuticle cells, in evaluating or screening cosmetics, skin external preparations, or the like using three-dimensional cultured skin model.SOLUTION: The observation method of cuticle cells includes removing the top surface of an epithelium reconstructed body and observing the epithelium reconstructed body with a microscope. Preferably, the top surface of the epithelium reconstructed body is removed by tape-stripping, and a confocal laser microscope is used as the microscope.SELECTED DRAWING: Figure 7COPYRIGHT: (C)2017,JPO&INPIT【課題】 3次元培養皮膚モデルを用いた化粧料や皮膚外用剤等の評価やスクリーニングを行う際、より有用な角質細胞の観察方法を提供すること。【解決手段】 上皮再構築体の上面を除去し、上皮再構築体を顕微鏡で観察することを含む、角質細胞の観察方法である。好ましくは、前記上皮再構築体の上面の除去はテープストリッピングで行い、顕微鏡は共焦点レーザー顕微鏡を用いる。【選択図】図7
