SYSTEM BASED ON PLASMIDS, METHOD TO PRODUCE A VIRUS OF VIRUSES WITH NEGATIVE RNA TAPE, METHOD TO PRODUCE A VIRION ORTHOMYXOVIRIDAE, METHOD TO PRODUCE A VIRION INFLUENZA, METHOD OF PRODUCING A VIRUS, TO INFERNATE A VIRACTION AND METHOD FOR GENERATING AN ATTENUATED NEGATIVE TAPE RNA VIRUS
"DNA transfection system for the production of an infectious influenza virus". the present invention is based on the development of a dual promoter system (preferably a pol i / pol ii system) for efficient intracellular synthesis of viral rna. the resulting system, based on a minimum number of plasmids, can be used to synthesize any RNA virus, preferably viruses with a negative strand RNA genome. the viral product of the system is produced when the plasmids in the system are introduced into an appropriate host cell. one application of the system is the production of the attenuated influenza virus reordered for use as an antigen in vaccines. the reordered viruses produced by co-transfection of plasmids can comprise genes encoding the surface of glycoprotein hemagglutinins and neuraminidase from an influenza virus currently infecting the population and endogenous genes from an attenuated influenza virus. an advantageous property of the present invention is versatility； the system can be quickly and easily adapted to synthesize an attenuated version of any rna virus. the attenuated or inactivated viral viruses produced by the present invention can be administered to a patient in need of a vaccine through any of several routes, including administration intranasally or intramuscularly."sistema de transfecção de dna para produção de um vírus influenza infeccioso". a presente invenção está baseada no desenvolvimento de um sistema promotor duplo (preferivelmente um sistema rna pol i/pol ii) para a síntese intracelular eficiente do rna viral. o sistema resultante, baseado em um número mínimo de plasmídeos pode ser usado para sintetizar qualquer vírus rna, preferivelmente, viroses com um genoma rna de fita negativa. o produto viral do sistema é produzido quando os plasmídeos do sistema são introduzidos dentro de uma célula hospedeira apropriada. uma aplicação do sistema é a produção do vírus influenza atenuado reordenado para uso como antígeno em vacinas. as viroses reordenad
