method for treating an individual in need of a non-syngeneic cell graft or tissue, method for treating an individual in need of an immature hematopoietic cell transplantation, method for inducing donor specific tolerance in an individual in need of a cell non-syngeneic or tissue graft, and method for treating an individual who needs a non-syngeneic cell or tissue graft
A method of treating a subject in need of a non-syngeneic cell or tissue graft is disclosed. The method comprising: (a) transplanting into a subject a dose of T cell depleted immature hematopoietic cells, wherein the T cell depleted immature hematopoietic cells comprise less than 5×105 CD3+ T cells per kilogram body weight of the subject, and wherein the dose comprises at least about 5×106 CD34+ cells per kilogram body weight of the subject； and subsequently (b) administering to the subject a therapeutically effective amount of cyclophosphamide, wherein the therapeutically effective amount comprises 25-200 mg per kilogram body weight, thereby treating the subject.
