PROBLEM TO BE SOLVED: To provide a base material for DANCE protein-containing tissue regeneration, capable of stably holding DANCE protein, a method of manufacturing the same, and a method of manufacturing cultured skin containing elastic fibrous tissue using the base material, or the like.SOLUTION: In the method of manufacturing the base material for the DANCE protein-containing tissue regeneration or the like, crosslinked collagen sponge is impregnated with a DANCE protein containing collagen solution and it is freeze-dried thereafter.