Amir Avniel,Efrat Lidor-Nili,Rudy Maor,Ofir Meir,Orly Noivirt-Brik,Osnat Yanai-Azulay
申请号：
US14143748
公开号：
US10041068B2
申请日:
2013.12.30
申请国别(地区):
US
年份:
2018
代理人:
摘要：
An isolated dsRNA molecule comprising an antisense RNA sequence for regulating a target gene of interest in a plant or a phytopathogen of the plant, wherein the dsRNA sequence is flanked by two complementary sites to an smRNA or smRNAs expressed in the plant and wherein the dsRNA molecule further comprises a helicase binding site positioned so as to allow unwinding of the strands of the isolated dsRNA molecule to single stranded RNA (ssRNA) and recruitment of an RNA-dependent RNA polymerase so as to amplify the dsRNA molecule in the plant cell and generate secondary siRNA products of the dsRNA sequence.
